Sequentially fermentation of traditional Chinese medicine by Pichia kudriavzevii and bifidobacteria for Skin Care Function

Abstract In order to explore the feasibility on Skin Care Function of fermentative traditional Chinese medicine, Pichia kudriavzevii strains isolated from Strong-flavor baijiu grains were used to ferment food homologous traditional Chinese medicine with the assistance of bifidobacterial. In the present study, we analyzed 16 Pichia kudriavzevii strains and 13 kinds of Chinese herbal medicines. Firstly, 9 Chinese herbal medicines were selected according the growth of bifidobacteria in the broth of 13 Chinese herbal medicines; then 9 Pichia kudriavzevii strains that could promote the growth of bifidobacteria were screened by the method of sequential fermentation in the 9 Chinese herbal medicines; after that, 9 Pichia kudriavzevii strains and bifidobacteria sequentially fermented the broth composed of the above 9 kinds of traditional Chinese medicine according to different formulas, and the moisture residual rate, hydroxyl radical scavenging rate and tyrosinase inhibition rate of the broth were detected to evaluate its skin care function. Better skin care function was found in the sequentially fermented broth of No.3 composed of Rhodiola, Raspberry, Bletilla striata, Atractylodes by YB07 and bifidobacterial, which displayed the moisture residual rate of 6.36%, the hydroxyl radical scavenging rate of 83.95% and the tyrosinase inhibition rate of 60.3%. Proteomic analysis by LC-MS showed this broth contained 99 active peptides (＜1000 Da) in skin lotion. 24h skin patch and 30-day skin safety test preliminarily revealed the safety of the fermentation broth as skin care products. This study provides a new perspective of food homologous traditional Chinese medicine for skin care products.