Sequentially fermentation of traditional Chinese medicine by Pichia kudriavzevii and bifidobacteria for Skin Care Function

Abstract To explore the feasibility of the Skin Care Function of fermentative traditional Chinese medicine, Pichia kudriavzevii strains isolated from Strong-flavor baijiu grains were used to ferment food homologous traditional Chinese medicine with the assistance of bifidobacterial. We analyzed 16 Pichia kudriavzevii strains and 13 kinds of Chinese herbal medicines in the present study. Firstly, nine Chinese herbal medicines were selected according to the growth of bifidobacteria in the broth of 13 Chinese herbal medicines. The 9 Pichia kudriavzevii strains that could promote the growth of bifidobacteria were screened by sequential fermentation. The 9 Pichia kudriavzevii strains and bifidobacteria sequentially fermented the above nine kinds of traditional Chinese medicine broth according to different formulas. The broth's residual moisture rate, hydroxyl radical scavenging rate, and tyrosinase inhibition rate were detected to evaluate its skincare function. Better skin care function was found in the sequentially fermented broth of No.3 composed of Rhodiola, Raspberry, Bletilla striata, Atractylodes by YB07, and bifidobacterial, which displayed the moisture residual rate of 46.36%, the hydroxyl radical scavenging rate of 83.95% and the tyrosinase inhibition rate of 60.3%. Proteomic analysis by LC-MS showed that this broth contained 99 active peptides (＜1000 Da) in skin lotion. 24h skin patch and 30-day skin safety test preliminarily revealed the safety of the fermentation broth as a skin care product. This study provides a new perspective on food homologous traditional Chinese medicine for skin care products.