Designing and optimizing 3D printed supports for paper microfluidic organ-on-chip devices

Microfluidics has become a foundation for organ-on-chip and paper-based analytical models. Creating reliable support structures are essential for high-performance microfluidic devices and creating them still remains as a challenge. This study presents the design, 3D printing, and evaluation of microfluidic device support, focusing on 3D material printing properties, structural stability and flow control in paper-based organ-on-chip platforms. Various geometries were tested, and printing parameters-layer thickness, exposure time, and speed—were optimized using photopolymer resin. The designed and printed microfluidic supports were tested for dimensional accuracy and flow performance. Optimized printing conditions produced supports that closely matched design specifications, provided robust structural support, and ensured consistent microfluidic flow. Obtained results highlight the importance of carefully considering the design and precision of 3D printing settings that offer scalable and practical approach regarding integration of the 3D-printed supports into organ-on-chip and paper-based microfluidic devices, all of which are important for supporting reproducible and efficient biomedical experiments.