Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress
View through CrossRef
Abstract
BackgroundCysteine protease (CP) plays an important role in plant senescence. In this study, the whole genome evolution information of CPs was analyzed by using safflower, and the function of CtCP1 under l abiotic stress was analyzed.Results25 CPs members were identified in the safflower genome and divided into 9 subfamilies. Gene structure analysis showed that the possible evolutionary conservatism and functional similarity of the same family members. qRT-PCR at different florescence showed that the expression of CPs gene was the highest in the decline period, and CtCP1 gene changed significantly under abiotic stress. We cloned the qRT-PCR of CtCP1, at different florescence and stress, which showed that the expression of CtCP1 was the highest in the decline stage and low temperature. In order to study the function of CtCP1 gene, we obtained the overexpression CtCP1 line (OE) and the inhibition CtCP1 expression line (Anti) in Arabidopsis thaliana. The results of transgenic lines under low temperature stress showed that inhibition of CtCP1 expression enhanced the resistance of Carthamus tinctorius to low temperature, and overexpression of CtCP1 weakened the resistance of Carthamus tinctorius to low temperature.ConclusionWe have identified the cysteine protease genome of safflower and CtCP1 gene expression under abiotic stress
Springer Science and Business Media LLC
Title: Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress
Description:
Abstract
BackgroundCysteine protease (CP) plays an important role in plant senescence.
In this study, the whole genome evolution information of CPs was analyzed by using safflower, and the function of CtCP1 under l abiotic stress was analyzed.
Results25 CPs members were identified in the safflower genome and divided into 9 subfamilies.
Gene structure analysis showed that the possible evolutionary conservatism and functional similarity of the same family members.
qRT-PCR at different florescence showed that the expression of CPs gene was the highest in the decline period, and CtCP1 gene changed significantly under abiotic stress.
We cloned the qRT-PCR of CtCP1, at different florescence and stress, which showed that the expression of CtCP1 was the highest in the decline stage and low temperature.
In order to study the function of CtCP1 gene, we obtained the overexpression CtCP1 line (OE) and the inhibition CtCP1 expression line (Anti) in Arabidopsis thaliana.
The results of transgenic lines under low temperature stress showed that inhibition of CtCP1 expression enhanced the resistance of Carthamus tinctorius to low temperature, and overexpression of CtCP1 weakened the resistance of Carthamus tinctorius to low temperature.
ConclusionWe have identified the cysteine protease genome of safflower and CtCP1 gene expression under abiotic stress.
Related Results
Growth and development of Carthamus tinctorius L. in the conditions of Transnistria
Growth and development of Carthamus tinctorius L. in the conditions of Transnistria
Carthamus tinctorius L. is a plant, that is used for oil production and dyeing, and also used for medical purposes and cosmetology. A distinctive biological feature of this plant i...
Evaluation of Anti-Diabetic Activity of Novel Bioactive Compounds of Carthamus Tinctorius Linn
Evaluation of Anti-Diabetic Activity of Novel Bioactive Compounds of Carthamus Tinctorius Linn
In the present study normal and alloxan-diabetic rats were used to evaluate the hypoglyceamic activities of the Carthamus tinctorius Linn extract and isolated compounds. Extraction...
AUTOPHAGY CONTROLS EPITHELIAL PROTEOLYTIC HOMEOSTASIS OF THE INTESTINAL MUCOSA
AUTOPHAGY CONTROLS EPITHELIAL PROTEOLYTIC HOMEOSTASIS OF THE INTESTINAL MUCOSA
Background
Crohn's Disease (CD) is a chronic relapsing inflammatory bowel disease (IBD) with mucosal ulcerations affecting all of the digestive tract. Intestina...
Microrna Regulation of Nodule Zone-Specific Gene Expression In Soybean
Microrna Regulation of Nodule Zone-Specific Gene Expression In Soybean
Nitrogen is a paramount important essential element for all living organisms. It has been found to bea crucial structural component of proteins, nucleic acids, enzymes and other ce...
Abiotic stress in plants: From Genomics to Metabolomics
Abiotic stress in plants: From Genomics to Metabolomics
Plants are exposed to various abiotic stresses such as water deficit or ion excess, elevated temperature,
high light intensity, salinity, freezing, cold, etc. under field condition...
Phylogenetic analysis of Carthamus species based on the nucleotide sequence of the nuclear SACPD gene and chloroplast trnL–trnF IGS region
Phylogenetic analysis of Carthamus species based on the nucleotide sequence of the nuclear SACPD gene and chloroplast trnL–trnF IGS region
To clarify the phylogenetic relationships of Carthamus species, we performed sequence analysis of the nuclear stearoyl acyl carrier protein desaturase (SACPD) gene and the chloropl...
Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent
Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent
Abstract
BackgroundA wide variety of Bacterial species produces protease enzyme and the application of same enzyme have been manipulated precisely and used in various biote...
Drug resistance during indinavir therapy is caused by mutations in the protease gene and in its Gag substrate cleavage sites
Drug resistance during indinavir therapy is caused by mutations in the protease gene and in its Gag substrate cleavage sites
Two different responses to the therapy were observed in a group of patients receiving the protease inhibitor indinavir. In one, suppression of virus replication occurred and has pe...