Identifikasi Kemampuan Bakteri dari Rizosfer Kakao dalam Menghambat Phytophthora palmivora Secara In Vitro

Abstrac. Phytophthora palmivora merupakan penyakit yang menyebabkan kerugian besar. Pengendalian terbaik yang harus diupayakan saat ini yaitu menggunakan pengendalian hayati dengan mengisolasi bakteri di daerah rizosfer tanaman kakao. Tujuan dari penelitian ini untuk mendapatkan isolat rizobakteri yang mampu berperan sebagai kadidat agen biokontrol. Penelitian dilaksanakaan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh dari bulan Maret sampai Juni 2018. Rancangan Acak Lengkap (RAL) non Faktorial adalah rancangan yang digunakan dalam penelitian ini dengan parameter pengamatan yang diamati adalah persentase pengahambatan pertumbuhan koloni patogen dan laju pengahambatan pertumbuhan koloni patogen. Daya hambat isolat bakteri yang diperoleh dari rizosfer kakao terbaik ditemukan pada isolat TRIM8/9 dengan persentase 76,67% dan laju hambat 3,16 mm/hari.Identification of the Bacterial Ability of Rizosfer Cocoa in Inhibiting Phytophthora palmivora in In VitroAbstract. Phytophthora palmivora is a disease that causes large losses. The best control that must be sought now is to use biological control by isolating rhizobacteria in the rhizosphere of the cocoa plant. The purpose of this study was to obtain bacterial isolates who were able to act as candidates for biocontrol agents. The research was conducted at the Seed Science and Technology Laboratory of the Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh from March to June 2018. Non Factorial Randomized Complete Design (CRD) was the design used in this study with observed observation variables as percentage inhibition of growth of pathogenic colonies and the rate of inhibition of growth of pathogenic colonies. The inhibition of bacterial isolates obtained from the best cocoa rhizosphere was found in TRIM8/9 isolates with a percentage of 76.67% and inhibition rate of 3.16 mm / day.