Optimizing Photoautotrophic Micropropagation Conditions for Ginger

Abstract Ginger (Zingiber officinale), a member of the Zingiberaceae family, is a commonly available spice and medicinal plant. This study aimed to develop an alternative conventional micropropagation using photomixotrophic micropropagation for ginger. Rootless ginger tissue culture plantlets were initiated in culture vessels grown a chamber maintained under a 16 h/day photoperiod with an intensity of 60 μE m-2 s-1 from cool white fluorescent lamps at 25 ± 2℃ with an air relative humidity of 60 ± 5%. Photoautotrophic micropropagation of ginger was optimized using vermiculite + peat (1:1(v/v)) as substrate combined with MS + 0.5 mg/L NAA + 0.1% Metalaxyl-M·Hymexazol + 80% relative humidity (RH). After 40 days of culture, in-vitro ginger seedlings were successfully bred with a rooting rate of 100.0%.