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Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum
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The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO----CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO. Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.
American Society for Microbiology
Title: Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum
Description:
The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions.
CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO.
Overall product stoichiometry approximated 6.
5CO----CH3CO2H + 3.
5CO2 + 0.
6 cell C + 0.
5 unrecovered C.
Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields.
Maximal YCO values approximated 2.
5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO.
Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.
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