e0106 The effect of adenosine and ischaemia postconditioning on MMP-2 and MMP-9 in rabbit ischaemia reperfusion myocardial

Objective To observe the effect of adenosine and ischaemia postconditioning on MMP-2 and MMP-9 in rabbit ischaemia reperfusion myocardial. Methods The rabbits were divided into four groups in basic experiment: control group, antagon group, postconditiong group and adenosine group. The activity of MMP-2 and MMP-9 was observed by gelatin zymography and the expression of MMP-2 and MMP-9 was observed by RT-PCR and Western Blot. Results The results of RT-PCR showed that the light density of MMP-2/GAPDH (0.76±0.22) in adenosine group and postconditioning group was slightly lower than that of control group and antagon group0. 80±0. 20 (p>0.05). The light density of MMP-2/GAPDH (0.77±0.12) in adenosine group and postconditioning group was significanty lower than that of control group and antagon group 1.30±0. 10 (p<0.01). There was no dramitic difference between adenosine group and postconditioning group. The results of Western blot showed that MMP-9 and MMP-2 in adenosine group and postconditioning group was lower than control group and antagon group. The results of Zymography revealed that the light density of MMP-9 in adenosine group and postconditioning group much lower than those in control group and antagon group (p<0.05). There was no difference between adenosine group and postconditioning (p>0.05). Conclusions Adenosine and postconditioning can decrease the expression and the activity of MMP-9 and inhibit the inflammation, relieving the ischaemia reperfusion injury.