e0618 Effects of Rosuvastatin on plasma NO and ET-1 during myocardial ischaemia-reperfusion injury in rabbits

Objective To study the change of endothelial function during myocardial ischaemia-reperfusion injury in rabbits and the effect of Rosuvastatin. Methods 16 New Zealand rabbits were randomLy divided into two groups: ischaemia/reperfusion injury group (control group) and Rosuvastatin group (drug group). Establish the myocardial ischaemia-reperfusion model. The camponotus upward elevation (≥0.2 mv) of the ST segment shown by the ECG indicated the successful ligation of the left anterior descending coronary artery; 40 mins later the ligation line was cut off, and the ST segment of ECG returned to 1/2 or more, which showed the success of reperfusion. At the four time points, before occlusion, 40 min after occlusion, 60 mins and 180 mins after reperfusion. We measured rabbit serum nitric oxide (NO), plasma endothelia-1 (ET-1) content. SPSS 11.5 software was applied, using ANOVA to p <0.05 for differences with statistical significance. Results In both groups, the serum NO content reduced gradually and the plasma ET-1 content increased gradually with protraction of the ischaemia and reperfusion time. Before ischaemia the serum NO[(109.875±32.255) μmol/l vs (114.500±37.405) μmol/l, p>0.05] and plasma ET-1 [(221.111±28.125) pg/ml vs (204.594±31.790) pg/ml, p>0.05], have no significant difference between the groups. At other three time points, the increased serum NO content [(63.125±18.962), (43.500±16.518), (29.625±14.162) μmol/l vs (82.000±13.825), (63.375±17.541), (50.250±18.987) μmol/l, p<0.05] in drug group was markedly lower than that in the control group and the reduced plasma ET-1 content [(331.785±35.341), (375.914±45.204), (459.829±70.110) pg/ml vs (282.541±38.928), (315.152±55.263), (377.795±60.427) pg/ml, p<0.05] in drug group was markedly higher than those in the control group. Conclusions Through increasing serum NO, and reducing plasma ET-1, rosuvastatin can improve endothelial function in myocardial ischaemia-reperfusion injury rabbits.