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Internal Epitope Tagging of Proteins Using Transposon Tn7 v1
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This is a protocol for in vitro transposon mutagenesis for introduction of internal epitope tags. It is from: Zordan RE, Beliveau BJ, Trow JA, Craig NL, and Cormack BP (2015) Avoiding the Ends: Internal Epitope Tagging of Proteins Using Transposon Tn7. Genetics 200:47-58; doi:10.1534/genetics.114.169482 Please see the full manuscript for additional details. This protocol delineates the steps to perform a small (20μl) “‐enzyme” negative control reaction and a large (80μl) “+ enzyme” experimental mutagenesis reaction in parallel. The enzymes and buffers are mixed together in a master mix (5.5‐reaction sized, to account for pipetting error) and later split into appropriate reaction sizes.
Title: Internal Epitope Tagging of Proteins Using Transposon Tn7 v1
Description:
This is a protocol for in vitro transposon mutagenesis for introduction of internal epitope tags.
It is from: Zordan RE, Beliveau BJ, Trow JA, Craig NL, and Cormack BP (2015) Avoiding the Ends: Internal Epitope Tagging of Proteins Using Transposon Tn7.
Genetics 200:47-58; doi:10.
1534/genetics.
114.
169482 Please see the full manuscript for additional details.
This protocol delineates the steps to perform a small (20μl) “‐enzyme” negative control reaction and a large (80μl) “+ enzyme” experimental mutagenesis reaction in parallel.
The enzymes and buffers are mixed together in a master mix (5.
5‐reaction sized, to account for pipetting error) and later split into appropriate reaction sizes.
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