Discover of No-Side Effect Phenytoin Drug from Catharanthus Roseus Plant

Abstract Phenytoin drug is commonly used for seizures and breast cancer, but chemically prepared phenytoin drug has more side effect. Herein, we have discovered phenytoin drug (100%) without side effect from Catharanthus Roseus (CR) plant using a photon-induced method (PIM). The pure plant (absence of metal nanoparticles) synthesized by green chemistry offers a novel and potential alternative to non-chemically synthesized Catharanthus Roseus nano-sized particles (CR-NPs,) studied for the first time. The GC-MS analysis of CR-NPs provides all peaks determining the presence of phenytoin compound and 1H NMR analysis of chemical shift conformed phenytoin structure. The XRD analysis results in highly crystalline material and crystallite size is 25 nm. The CR NPs highly killed breast cancer cells (MCF-7 cell-lines), while without killed the normal cells (VERO cell-lines). Our findings brought new insights into the development of nano-sized plant exerting strong next generation of no-side effect nature drug.