Protective Effect of Ethanol Extract of Vanda tessellata Leaves on Methotrexate Induced Hepatotoxicity and Nephrotoxicity in Rats

Drugs and chemicals are considered as one of the major causes for hepatotoxicity and renal toxicity, inducing direct damage through multiple pathways including oxidative stress. The present study was aimed to investigate the protective effect of ethanol extract of Vanda tessellata leaves in methotrexate induced hepatotoxicity and nephrotoxicity rats. The ethanol extract of Vanda tessellata leaves were prepared and its protective effect were evaluated for methotrexate induced liver and kidney damaged in rats. The administration of ethanol extract of Vanda tessellata (150 and 300 mg/kg) significant (p<0.05) decrease in serum SGOT, SGPT, ALP, ACP and total bilirubin compared to control group rats treated MTX. Treating with ethanol extract of Vanda tessellata showed significant decrease (p<0.05) in concentration of serum urea, Creatinine, Uric acid, Total protein and BUN compared to MTX treated groups. The findings of biochemical parameters illustrated that the ethanol extract of Vanda tessellata leaves showed protective properties against MTX induced hepatotoxicity and nephrotoxicity in rats.