Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Sequential extraction and immunoblotting v1
View through CrossRef
This protocol examines the fraction of alpha-synuclein (as assessed by alpha-synuclein and/or PS129 western blot) that is present in the triton-soluble or SDS-soluble fraction. Addition of alpha-synuclein pre-formed fibrils (PFFs) to neuron cultures seeds the recruitment of endogenous or transgenic alpha-synuclein into aggregates characterized by detergent insolubility. Monomeric alpha-synuclein will be present in the triton-soluble fraction, whereas PFF-induced alpha-synuclein oligomers will be present in the triton-insoluble, SDS-soluble fraction. This protocol encompasses preparation of cell extracts with Triton X-100, followed by sequential extraction of the Triton-insoluble material with SDS. The protein in the different detergent fractions are quantified by BCA, followed by SDS-PAGE and WB for alpha-synuclein, PS129, TUJ1, and loading control such as GAPDH.
ZappyLab, Inc.
Title: Sequential extraction and immunoblotting v1
Description:
This protocol examines the fraction of alpha-synuclein (as assessed by alpha-synuclein and/or PS129 western blot) that is present in the triton-soluble or SDS-soluble fraction.
Addition of alpha-synuclein pre-formed fibrils (PFFs) to neuron cultures seeds the recruitment of endogenous or transgenic alpha-synuclein into aggregates characterized by detergent insolubility.
Monomeric alpha-synuclein will be present in the triton-soluble fraction, whereas PFF-induced alpha-synuclein oligomers will be present in the triton-insoluble, SDS-soluble fraction.
This protocol encompasses preparation of cell extracts with Triton X-100, followed by sequential extraction of the Triton-insoluble material with SDS.
The protein in the different detergent fractions are quantified by BCA, followed by SDS-PAGE and WB for alpha-synuclein, PS129, TUJ1, and loading control such as GAPDH.
Related Results
Optimization of ultrasonic extraction of
Lycium barbarum
polysaccharides using response surface methodology
Optimization of ultrasonic extraction of
Lycium barbarum
polysaccharides using response surface methodology
Abstract
Ultrasonic extraction was a new development method to achieve high-efficiency extraction of
Lycium barbarum
...
Utilizing Large Language Models for Geoscience Literature Information Extraction
Utilizing Large Language Models for Geoscience Literature Information Extraction
Extracting information from unstructured and semi-structured geoscience literature is a crucial step in conducting geological research. The traditional machine learning extraction ...
Sequential Speciation and Cascading Divergence
Sequential Speciation and Cascading Divergence
A central, long-standing, and largely untested premise in evolutionary ecology is that “biodiversity begets biodiversity” in a process referred to as “sequential” or “cascading” di...
Extraction of Mogroside and Limonin with Different Extraction Methods and its Modeling
Extraction of Mogroside and Limonin with Different Extraction Methods and its Modeling
The extraction yields of mogroside from Siraitia grosvenorii fruits and limonin from orange (Citrus reticulata Blanco) seeds were compared with different extraction methods, respec...
EWOD Based Liquid-Liquid Extraction and Separation
EWOD Based Liquid-Liquid Extraction and Separation
Liquid-liquid extraction techniques are one of the major tools in chemical engineering, analytical chemistry, and biology, especially in a system where two immiscible liquids have ...
Asymptotical problems of sequential interval and point estimation
Asymptotical problems of sequential interval and point estimation
The accuracy of interval estimation systems is usually measured using interval lengths for given covering probabilities. The confidence intervals are the intervals of a fixed width...
On δ-Sequential Spaces
On δ-Sequential Spaces
Sequence converges is an important research object in topology and analysis, since it is closely related to continuity, compactness and other related properties. In this article, w...
Response Surface Analysis on Multiple Parameter Effects on Borehole Gas Extraction Efficiency
Response Surface Analysis on Multiple Parameter Effects on Borehole Gas Extraction Efficiency
To explore the impact of different factors on the effectiveness of borehole gas extraction, in situ stress tests were conducted in a test mining area. A theoretical model of gas mi...