Subtyping of group‐specific component and protease inhibitor by rapid isoelectric focusing on PhastSystem

AbstractA rapid method on PhastSystem was used to investigate the distribution of group‐specific component (GC) and protease inhibitor (PI) subtypes and their gene frequencies from 190 unrelated healthy donors of the Han population in Beijing. Laboratory‐made gels (pH 4.5–5.4 and pH 4.2–4.9) were used for analysis of GC and PI, respectively. Sample loading was 1.5 μL. The separation and visualization time was 0.5 h in each. Gene frequencies were as follows: GC*1F = 0.4891, GC*1S = 0.2432, GC*2 = 0.2678; rare GC variants were discovered in seven cases. The results for PI were; PI*M1=0.7542, PI*M2 = 0.1808, PI*M3 = 0.0650. Good agreement between the observed and expected values in both GC and PI subtyping (for GC, ∑χ2 = 1.4043, 0.7< P < 0.8; for PI, ∑χ2 = 1.1233, 0.7 < P < 0.8) was obtained.