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Phenotyping of esterase D and acid phosphatase by rapid isoeletric focusing on PhastSystem
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AbstractA rapid isoelectric focusing method on PhastSystem was applied to investigate the distribution of esterase D (ESD) and acid phosphatase (ACP) phenotypes in 152 and 112 unrelated healthy donors, respectively, from the Han population in Beijing. Laboratory‐made gels with a pH range of 5.0–7.0 and 5.0–8.0 were used for analysis of ESD and ACP, respectively, with 1 μL sample loading. The running time was 30–40 min. Gene frequencies for ESD were 0.6809 for ESD*1 and 0.3191 for ESD*2, (∑χ2 = 0.8586 and P ≥ 0.50); gene frequencies of ACP were 0.2009 for ACP*A, 0.7991 for ACP*B, (∑χ2 = 1.7892 and P > 0.20), with good agreement between the observed and the expected values.
Title: Phenotyping of esterase D and acid phosphatase by rapid isoeletric focusing on PhastSystem
Description:
AbstractA rapid isoelectric focusing method on PhastSystem was applied to investigate the distribution of esterase D (ESD) and acid phosphatase (ACP) phenotypes in 152 and 112 unrelated healthy donors, respectively, from the Han population in Beijing.
Laboratory‐made gels with a pH range of 5.
0–7.
0 and 5.
0–8.
0 were used for analysis of ESD and ACP, respectively, with 1 μL sample loading.
The running time was 30–40 min.
Gene frequencies for ESD were 0.
6809 for ESD*1 and 0.
3191 for ESD*2, (∑χ2 = 0.
8586 and P ≥ 0.
50); gene frequencies of ACP were 0.
2009 for ACP*A, 0.
7991 for ACP*B, (∑χ2 = 1.
7892 and P > 0.
20), with good agreement between the observed and the expected values.
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