Optimization of Extraction Conditions of Active Constituents from Siraitia Grosvenorii

The conditions of cascade extraction and isolation of some active constituents from Siraitia grosvenorii were optimized. The active constituents were extracted by ultrasonic extraction and isolated by chromatography; the obtained active constituents were determined by HPLC and/or spectrophotometry etc. The yields of the active constituents were as follows, mogroside 3.97% (purity 91.84%), polyphenol 3.45%, free polysaccharide 12.53%, free oligosaccharide 11.96%, combined polysaccharide 2.29%, combined oligosaccharide 2.88%, amino acids 6.54% and alkaloid 7.06%. This extraction process could be used as a guideline for the multi-utilization of Siraitia grosvenorii.