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Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
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Zymomonas mobilis ZM4 is an attractive host for the development of
microbial cell factories to synthesize high-value compounds, including
prebiotics. In this study, a straightforward process to produce
fructooligosaccharides (FOS) from sucrose was established. To control
the relative FOS composition, recombinant Z. mobilis strains secreting a
native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase
(Ffase-Leu196) from Schwanniomyces occidentalis were constructed. Both
strains were able to produce a FOS mixture with high concentration of
6-kestose. The best results were obtained with Z. mobilis ZM4 pB1-sacB,
that was able to produce 73.4 ± 1.6 g L−1 of FOS, with a productivity of
1.53 ± 0.03 g L-1 h-1 and a yield of 0.31 ± 0.03 gFOS gsucrose-1. This
is the first report on the FOS production using a mutant Z. mobilis ZM4
strain in a one-step process.
Title: Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4
Description:
Zymomonas mobilis ZM4 is an attractive host for the development of
microbial cell factories to synthesize high-value compounds, including
prebiotics.
In this study, a straightforward process to produce
fructooligosaccharides (FOS) from sucrose was established.
To control
the relative FOS composition, recombinant Z.
mobilis strains secreting a
native levansucrase (encoded by sacB) or a mutated β-fructofuranosidase
(Ffase-Leu196) from Schwanniomyces occidentalis were constructed.
Both
strains were able to produce a FOS mixture with high concentration of
6-kestose.
The best results were obtained with Z.
mobilis ZM4 pB1-sacB,
that was able to produce 73.
4 ± 1.
6 g L−1 of FOS, with a productivity of
1.
53 ± 0.
03 g L-1 h-1 and a yield of 0.
31 ± 0.
03 gFOS gsucrose-1.
This
is the first report on the FOS production using a mutant Z.
mobilis ZM4
strain in a one-step process.
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