Potential Probiotic Lactobacillus Rhamnosus (MTCC-5897) Attenuates Escherichia Coli Induced Inflammatory Response in Intestinal Cells

Abstract Probiotics are microbes having tremendous potential to prevent gastrointestinal disorders. In current investigation, immunomodulatory action of probiotic Lactobacillus rhamnosus (LR:MTCC-5897) was studied during exclusion, competition and displacement of Escherichia coli on intestinal epithelial (Caco-2) cells. The incubation of intestinal cells with E. coli, enhanced downstream signalling and activated nuclear factor kappa B (NF-κB). This significantly increased (p<0.01) the pro-inflammatory cytokines (IL-8, TNF-α and IFN-ϒ) expression. While, incubation of epithelial cells with L. rhamnosus during exclusion and competition with E. coli, counteracted these enhanced expressions. The immunomodulatory feature of L rhamnosus was also highlighted with increased (p<0.05) transcription of toll like receptor-2 (TLR-2) and single Ig IL-1-related receptor (SIGIRR) along with diminished expression of TLR-4. Likewise, attenuation (p<0.05) of E.coli-mediated enhanced nuclear translocation of NF-κB p-65 subunit by L. rhamnosus during exclusion was confirmed with western blotting. Thus, present finding establishes the prophylactic potential of L. rhamnosus against exclusion of E. coli in intestinal cells.