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Molecular analysis of the aroA gene of Pasteurella multocida and vaccine potential of a constructed aroA mutant
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SummaryThe aroA gene from Pasteurella multocida was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18. The nucleotide sequence of the P. multocida aroA gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial AroA proteins. The cloned P. multocida aroA gene was inactivated by insertion of a kanamycin‐resistance gene and reintroduced by allelic exchange into the chromosome of P. multocida using the suicide vector pJM703.1. The P. multocida aroA mutant was highly attenuated in a mouse model. Mice immunized intraperitoneally with two doses of live P. multocida aroA mutant were completely protected against a lethal parental strain challenge.
Title: Molecular analysis of the aroA gene of Pasteurella multocida and vaccine potential of a constructed aroA mutant
Description:
SummaryThe aroA gene from Pasteurella multocida was cloned by complementation of the Escherichia coli aroA mutant AB2829 with a DNA library constructed in pUC18.
The nucleotide sequence of the P.
multocida aroA gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial AroA proteins.
The cloned P.
multocida aroA gene was inactivated by insertion of a kanamycin‐resistance gene and reintroduced by allelic exchange into the chromosome of P.
multocida using the suicide vector pJM703.
1.
The P.
multocida aroA mutant was highly attenuated in a mouse model.
Mice immunized intraperitoneally with two doses of live P.
multocida aroA mutant were completely protected against a lethal parental strain challenge.
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