Fibrinolytic Enzyme from Bacillus Pumilus Strain (MPMBJ1) through Solid-State Fermentation

Fibrinolytic enzymes are produced by several Bacillus species. For the therapeutic purpose, the fibrinolytic enzyme can be used as thrombolytic agent for the clot resolution. In the study, fibrinolytic enzymes are produced in the modified nutrient medium under solid-state fermentation using Bacillus pumilus strain-MPMBJ1 from CBNR isolate. The presence of fibrinolytic enzyme in the fermentation media was confirmed by salting-out methods in conjunction with SDS-PAGE. The enzyme was purified by using dialysis method followed by the chromatographic technique. Biological activities of the enzyme were assessed through proteolytic and fibrinolytic functional assays. The results showed that the optimum incubation period for fibrinolytic enzyme production is 24 hrs. We have obtained the yeilds of fibrinolytic enzyme as 64.55 ± 1.25 and 22.75 ± 0.75 mg/ml by using acetone and ammonium sulfate precipitation methods respectively. The molecular weight of the fibrinolytic enzyme was found to be 30kDa from SDS-PAGE analysis. The enzyme was found to exhibit potential proteolytic and fibrinolytic properties. Hence, further in vivo and clinical analysis on this enzyme may lead to the production of an effective drug for heart attack patients.