The Hematopoietic Effect of Red and White Peels Pomegranate Extract on Phenylhydrazine-Induced Anemia in Rats and LCMS/ MS investigations of new substances

Abstract Pomegranates, Punica granatum L., are said to provide various health advantages, according to accumulating research. The haematological effects of pomegranate white and red peel extracts on male rats with phenylhydrazine (PHZ)-induced anaemia are highlighted in this article. The phytoconstituents of the ethanolic extract of pomegranate white and red peels were identified using LCMS/MS analysis.We employed 48 male albino,. Six experimental groups of eight animals each were created at random from the total number of animals. Rats in group 1(C) served as the control, group, Group 2 (C-WPP), rats received WPP, Group 3 (C-RPP): rats received RPP extract, Group 4 (PHZ): rats received an i.p. injection of PHZ in saline in a dose of 50 mg/kg/day, Group 5 (PHZ-WPP) and, Group 6 (PHZ-RPP). Peripheral blood smears were measurements of iron level, G6PD, LDH, MDA, GPX, SOD, ALT, ALP, and AST were analyzed. By using LCMS/MS studies, 252 compounds were discovered; 163 of these compounds were tentatively recognized for the first time in the white and/or red peels of pomegranates, and 7 of these compounds were tentatively characterized for the first time in nature. Pretreatment with the white and/or red peels of pomegranates, however, greatly reduced this toxicity by modifying the organ's biomarkers, enhancing the tissue's redox status, and bringing serum biochemical parameters virtually back to normal levels. The ethanolic extract of red and white pomegranate peels displays a potent defense against free radicals and oxidative stress.