Evaluation of in- vitro Anti-inflammatory Potential of Grevillea robusta A. Cunn, EX R.BR. Leaves

Synthetic Non-steroidal anti-inflammatory drugs (NASAIDs) are the choice of drug for inflammation. NASAIDs caused severe side effects like hyperacidity, gastric ulcer and so on. To avoid the side effects of NASAIDs, there is an urgent need for searching new molecule from natural origin. Present study is therefore aimed to explore Grevillea robusta A. Cunn, ex R.Br. Family proteaceae leaves for anti-inflammatory activity. Microscopic measurement (fibre length and width), Ash values and extractive values of Grevillea robusta leaves were determined to set the pharmacognostic standards. Chemical constituents were evaluated through chemical tests. The Ethanol extract of Grevillea robusta leaves (GRLE)) were subjected to evaluate in-vitro anti-inflammatory activity through HRBC method and Heat induced haemolytic method. The leaves of Grevillea robusta showed significant anti-inflammatory activity. The Ethanol extract (GRLE) showed significant anti-inflammatory activities. GRLE was found to contain polyphenols as chemical constituents which was the basis of anti-inflammatory activity. On the basis of result we can conclude that Ethanol extract of leaves of Grevillea robusta has good anti-inflammatory activity. GRLE could be used for treatment of inflammation.