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Detection of microcystin-producingMicrocystisin Guanqiao Lake using a sandwich hybridization assay
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Based on sequence analyses of the mcyJ gene from Microcystis strains, a probe pair TJF and TJR was designed and a sandwich hybridization assay (SHA) was established to quantitatively detect microcystin-producing Microcystis. Through BLAST and cyanobacterial culture tests, TJF and TJR were demonstrated to be specific for microcystin-producing Microcystis. A calibration curve for the SHA was established, and the lowest detected concentration was 100 cells·mL–1. Laboratory cultures and field samples from Guanqiao Lake were analyzed with both the SHA and microscopy. The cell number of microcystin-producing Microcystis and that of total Microcystis were compared. The biotic and abiotic components of the samples were of little disturbance to the SHA. In this study, a SHA was established to detect Microcystis, providing an alternative to PCR–ELISA and real-time PCR technology.
Title: Detection of microcystin-producingMicrocystisin Guanqiao Lake using a sandwich hybridization assay
Description:
Based on sequence analyses of the mcyJ gene from Microcystis strains, a probe pair TJF and TJR was designed and a sandwich hybridization assay (SHA) was established to quantitatively detect microcystin-producing Microcystis.
Through BLAST and cyanobacterial culture tests, TJF and TJR were demonstrated to be specific for microcystin-producing Microcystis.
A calibration curve for the SHA was established, and the lowest detected concentration was 100 cells·mL–1.
Laboratory cultures and field samples from Guanqiao Lake were analyzed with both the SHA and microscopy.
The cell number of microcystin-producing Microcystis and that of total Microcystis were compared.
The biotic and abiotic components of the samples were of little disturbance to the SHA.
In this study, a SHA was established to detect Microcystis, providing an alternative to PCR–ELISA and real-time PCR technology.
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