Effect of Kitolod (Isotoma Longiflora) Leaf Extract Concentration in Gel Formula on Total Phenolic and Flavonoid Levels

Belonging to the Campanulaceae family, Kitolod (Isotoma longiflora) possesses antioxidant properties crucial for wound healing; presenting a case for its formulation into a gel and investigating the total phenolic and flavonoid content of kitolod leaf extract when in gel form is essential to understand its capabilities as an anti-inflammatory and wound-healing gel, as well as to examine the possible correlation between the extract's concentration and its phenolic and flavonoid content. This study assessed how different concentrations of kitolod leaf extract affect the total phenolic and flavonoid levels in a gel formulation. Using 96% ethanol, kitolod leaves were macerated. The gel formulations contained varying concentrations of kitolod leaf extract: F1(5%), F2(10%), and F3(20%). The presence of phenols and flavonoids was confirmed through qualitative tests using FeCl3 1% for phenols and the Shinoda test for flavonoids. The findings confirmed that the total levels of phenolics and flavonoids increased with the concentration of kitolod leaf extract in the gel. SPSS analysis showed a strong correlation between extract concentration in the gel and the amounts of total phenolics and flavonoids, highlighting a positive relationship between these two chemical compounds since they are in the same category.