Evaluation of Anti-inflammatory activity of Jacquemontia caerulea Leaf Methanolic Extract in Wistar rats

Objective: To evaluate anti-inflammatory activity of methanolic extract of obtained from the leaves of plant, Jacquemontia caerulea in Wistar rats. Methods: Fresh leaves of Jacquemontia caerulea (Family: Convolvulaceae) were extracted using methanol and subjected to phytochemical analysis using standard procedures. The anti-inflammatory nature of the methanolic leaf extract (JCLME) was assessed on Wistar rats, with Indomethacin serving as the standard reference drug. Two established methods were employed: Paw edema method by using Carrageenan and Cotton pellet granuloma method. Results: Phytochemical analysis of methanolic extract was found to have phytoconstituents such as saponins, flavonoids, glycosides, steroids, proteins, amino acids, phenols, and tannins. The anti-inflammatory evaluation showed that JCLME produced anti-inflammatory effect. In the paw edema model by Carrageenan, JCLME demonstrated remarkable (p<0.05) inhibition of paw edema with reduction of 23% and 30% at dose of 250 mg/kg and 500 mg/kg, respectively. Similar result was observed in the Cotton pellet granuloma method. Conclusion: The leaf methanolic extract of Jacquemontia caerulea contains a high concentration of various phytochemicals and exhibits significant (p<0.05) anti-inflammatory effect in both Carrageenan-induced paw edema and Cotton pellet granuloma model in Wistar rats.