ANTIBACTERIAL TEST OINTMENT PATIKAN KEBO LEAF EXTRACT (Euphorbia hirta L.) AGAINST Staphylococcus epidermidis BACTERIA USING DIFFUSION METHOD

Staphylococcus epidermidis is a microbe that causes skin infections (acne). Infection can be overcome with the use of natural antibiotics. Previous studies have shown that patikan kebo plants with concentrations of 0,5%, 1%, 2%, 4%, 8%, have active antimicrobials. The preparation of starch kebo leaf extract ointment is made with 3 variations in concentration (5%, 10%, and 15%) with flavonoids, triterpenoids, alkaloids, and tannins. The purpose of this study was to determine whether the preparation of patikan kebo leaf extract ointment (Euphorbia hirta L.) has antibacterial activity against Staphylococcus epidermidis and determine the physical quality of the ointment preparation. The method used is experimental laboratory which is useful to determine the effect of patikan kebo leaf extract with concentrations of 5%, 10%, and 15% on Staphylococcus epidermidis bacteria in ointment preparations. Antibacterial tests are carried out using the sumuran diffusion method. Antibacterial test results with concentrations of 5%, 10%, and 15% respectively are 9,00; 10,90 ; 13,60 mm has an influence on the inhibitory zone in Staphylococcus epidermidis bacteria. The results of the physical quality test found that formula 3 with a concentration of 15% has the highest bacterial inhibitory influence and has the longest adhesion.Analysis of the results of the physical quality evaluation of the adhesion test of ointment preparations showed a significant difference. While the dispersion test and viscosity test showed no significant difference.