(Invited) Wearable Flexible Microfluidic Biosensor for Sweat Monitoring

As we enter the post-pandemic era, our lives have certainly become more convenient than before, due to the advancement and elimination of technologies that became practical or widespread during the pandemic. Among these, technologies related to non-invasive bioinstrumentation, which can be used at an individual level to access one's own health information, have become increasingly important. Non-invasive bioinstrumentation can be broadly divided into the measurement of physical information (shape, activity and so on) and the measurement of chemical information, i.e. the components of the body. For example, facial shape is widely used for personal identification, while weight and energy expenditure related to activity are easily managed by smartphones. In contrast, chemical sensing, which is often difficult to sample and requires a wet environment for measurement, still requires breakthroughs to be used by individuals with the same ease as physical sensing. In the research field of non-invasive biomedical measurements, the technology is often developed in the form of wearable systems. Wearable microfluidic devices have been intensively studied for the measurement of body components. We focused on sweat, which contains a variety of biomarkers, and have developed a wearable microfluidic biosensing system that measures sweat components in real-time. Sweat is secreted from sweat glands on the skin of the entire body, making it easily accessible and less mentally demanding to collect. On the other hand, there are problems with sampling methods for reliable measurement, such as large fluctuations in the sweat rate and evaporation from the skin surface during secretion. We overcame this problem by using a wearable microfluidic device to transport the full amount of sweat from the sweat gland to a downstream biosensor by carrier flow, and developed a wristwatch biosensing system to monitor the constituents released as sweat independent of sweat rate. The wristwatch sweat sensor consists of a PDMS sweat sampling device, a biosensor, a fluid controller and a wireless control circuit with Bluetooth Low Energy and external analogue I/O. Communication with the mobile device is via Bluetooth Low Energy. Our method has the advantage that secretions are transported by dissolution in the carrier stream and can therefore be reliably monitored even at very low sweat levels. The pump is controlled by an microcontroller unit, so the flow rate can be controlled in real time according to the sweat rate and target substances, and the sensitivity can be improved at the expense of temporal resolution by running the pump intermittently, i.e. allowing secretions to accumulate in the carrier flow for a certain time before measurement. We monitored changes in sweat lactic acid in response to exercise load using an ergometer. The results showed an increase in sweat lactate with increasing exercise intensity, with a dependence on sweat rate. By measuring sweat at rest, we found that the lactate accumulated in the sweat glands and on the skin's surface were dissolved by the biosensor in the early stages of measurement, while the signals from the sweat glands became dominant after these had been sufficiently decreased. These results suggest that our system appropriately reflects the physiological phenomena associated with sweating. The presentation will also include the behaviour of lactic acid in sweat, which was detected by our system for the first time.