Genetic Variability Screening of the Leptolyngbya Boryana with Expressed ChrR Gene for the Biotransformation of Cr (VI) to Cr (III) Reduction

Abstract Chromium is well known pollutant for its mutagenicity, and carcinogenicity in humans. Excessive uses of chromium in leather tanning industries, stainless-steel production, and wood preservatives have resulted as chromium contamination in soil and water. This investigation indicates the effective use of Leptolyngbya boryana as an eco-friendly option to overcome Chromium (VI) toxicity in tannery effluents. The main objective of this research was to find out ChrR gene and its variability in the context of Cr (VI) stress. This is a novel study in the relation of Leptolyngbya boryana. Industrial polluted soil samples were collected and processed according to the standard protocols for ChrR variation and 16S rRNA gene. DNA was isolated and amplified through PCR. Amplified DNA was sequenced and aligned with the known sequences. In this study a strong co-relation was established in the nucleotide sequences of ChrR and 16S rRNA genes. MIC was determined for Cr (VI) and pure strains of Leptolyngbya boryana were identified and isolated from soil. In the present study presence of ChrR gene variability was recorded in Leptolyngbya boryana which is a cyanobacterium in the soil of tannery effluent under Cr (VI) stressed condition and its gene variability was confirmed by sequencing. We can conclude that Leptolyngbya boryana strain could be eco-friendly option to overcome Chromium (VI) toxicity in tannery effluents.