Dampak Pewarna Congo red terhadap Gambaran Histopatologi Berdasarkan Derajat Inflamasi

Abstract. Congo red is an azo dye widely used in the textile industry and has the potential to cause toxicity to male reproductive organs. This study aims to analyze the effect of congo red exposure on testicular histopathology. The study used a retrospective observational design with stored biological materials (SBM) consisting of 28 rat testis samples that had previously been exposed to Congo red at doses of 190 mg/kgBW, 375 mg/kgBW, and 750 mg/kgBW. The preparations were observed in 5 fields of view each, for a total of 140 preparations. The degree of inflammation was analyzed using the Kruskal-Wallis test. The results showed no inflammation in all groups (p = 1.000). It was concluded that Congo red exposure did not trigger inflammation. Abstrak. Congo red merupakan pewarna azo yang banyak digunakan pada industri tekstil dan berpotensi menimbulkan toksisitas terhadap organ reproduksi jantan. Penelitian ini bertujuan menganalisis pengaruh paparan congo red terhadap gambaran histopatologi testis. Penelitian menggunakan desain observasional retrospektif dengan Bahan Biologis Tersimpan (BBT) berupa 28 sampel testis tikus yang sebelumnya diberikan paparan congo red dosis 190 mg/kgBB, 375 mg/kgBB, dan 750 mg/kgBB. Preparat diamati pada masing-masing sebanyak 5 lapang pandang sehingga total 140 preparat. Derajat inflamasi dianalisis dengan uji Kruskal-Wallis. Hasil menunjukkan tidak terdapat inflamasi pada seluruh kelompok (p = 1.000). Disimpulkan bahwa paparan congo red tidak memicu inflamasi.