In vivo response of Tγδ cells against non-bilayer phospholipid arrangements 2858

Abstract Description   Systemic lupus erythematosus (SLE) is a chronic autoimmune condition characterized by diverse clinical presentations, with its onset mechanisms still largely unknown. Our research team suggests that SLE and related autoimmune disorders are linked to alterations in cell membranes, in which stable non-bilayer phospholipid arrangements (NPA) trigger the formation of autoantibodies. Therefore, this study analyzed the in vivo response of Tγδ cells to NPA in a mouse model of lupus induced by the administration of liposomes bearing NPA. We analyzed Tγδ cells from the spleen and mesenteric lymph nodes using flow cytometry to evaluate their activation, cell cycle, proliferation, cytokine production, and mitochondrial type at 5, 10, and 15 days post-NPA administration. Anti-NPA IgG antibodies were observed by day 5 post-injection of liposomes bearing NPA, with increasing levels over time. Tγδ cells in mice administered with liposomes showed a statistically significant rise in their total numbers throughout the study in the spleen and at day 10 in the mesenteric lymph nodes compared to those administrated with Tris-saline solution. These cells were primarily found in the S and G2 phases of the cell cycle, exhibited metabolic reprogramming due to mitochondrial fission, and predominantly produced IL-4 and IFNγ. Our results suggest that Tγδ cells are involved in the mechanisms that drive the adaptive immune response and may play a role in generating high-affinity anti-NPA antibodies. Funding Sources This study was supported by grants from Instituto Politécnico Nacional (SIP20241225, SIP20241230, and SIP20241234). Topic Categories Basic Autoimmunity (BA)