Production of Dextran from Domestic Weissella cibaria Isolated

Abstract Dextran is an exopolysaccharide (EPS) of bacterial origin that is readily available in the commercial market. that is used in various industrial applications as an adjuvant, emulsifier, carrier, and stabilizer in the food and biomedical industries. Industrial production of dextran is carried out by fermentation in sucrose-rich media. Studies to optimize dextran production have shown that dextran yield varies depending on specific production conditions. This study aimed to generate dextrans Lactobacillus species were obtained from the vaginal and stool samples of healthy infants. The mucosal technique was employed to determine the biosynthesis of dextran from various isolates of Lactobacillus spp. colony assay and confirmed by isopropanol precipitation. The effects of various parameters such as sucrose concentration, nitrogen concentration, temperature, incubation time, pH, and inoculum size were studied to optimize the maximum dextran yield. In the present study, Weissella cibaria may produce dextran. Optimal conditions for dextran production are a 48-hour incubation at The experimental conditions involved maintaining a temperature of 37°C, with a sugar concentration of 10%, and a yeast extract concentration of 0.5%. The inoculum volume was set at 4%, and the pH level was maintained at 6.5. The experiment was conducted under aerobic conditions. The obtained value for dextran dry weight was 520 mg/100 ml.