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Characterization of purified dextran from Lactobacillus fermentum
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The objective of the current study is to characterize dextran purified from Lactobacillus fermentum (Lb4) Isolates of Lactobacillus spp. were tested for dextran
production using mucoidy, ethanol precipitation and spectrophotometric method.
Results of the Spectrophotometric method showed that all isolates gave concentrations of dextran ranging from (0.1 to 0.2 mg/ml). Precipitation, purification and
characterization of dextran from L. fermentum (Lb4) were done. Thin-layer
chromatography (TLC), 1H Nuclear Magnetic Resonance (HNMR) Spectroscopy,
Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FE-SEM), Gas Chromatography-mass spectrometry (GC-MS),
Melting Point (MP), Water Solubility index (WSI), and Water Holding Capacity
(WHC) had been used for dextran characterization. Characterization proved that
pure dextran. Nanosphere structure with a nano-diameter range of about (56.00)
nm with molecular weight (73) kDa. According to the melting point test, the purified dextran from L. fermentum had been completely melted at 228°C. The water
solubility index (WSI) had been determined (93.35%) and the water holding
capacity (WHC) (300%).
Keywords: Lactobacillus spp. ; Dextran ; Lactobacillus fermentum
Clinical Biotec S.L.
Title: Characterization of purified dextran from Lactobacillus fermentum
Description:
The objective of the current study is to characterize dextran purified from Lactobacillus fermentum (Lb4) Isolates of Lactobacillus spp.
were tested for dextran
production using mucoidy, ethanol precipitation and spectrophotometric method.
Results of the Spectrophotometric method showed that all isolates gave concentrations of dextran ranging from (0.
1 to 0.
2 mg/ml).
Precipitation, purification and
characterization of dextran from L.
fermentum (Lb4) were done.
Thin-layer
chromatography (TLC), 1H Nuclear Magnetic Resonance (HNMR) Spectroscopy,
Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FE-SEM), Gas Chromatography-mass spectrometry (GC-MS),
Melting Point (MP), Water Solubility index (WSI), and Water Holding Capacity
(WHC) had been used for dextran characterization.
Characterization proved that
pure dextran.
Nanosphere structure with a nano-diameter range of about (56.
00)
nm with molecular weight (73) kDa.
According to the melting point test, the purified dextran from L.
fermentum had been completely melted at 228°C.
The water
solubility index (WSI) had been determined (93.
35%) and the water holding
capacity (WHC) (300%).
Keywords: Lactobacillus spp.
; Dextran ; Lactobacillus fermentum.
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