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The C‐terminal chromodomain‐like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY
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MAGGY is a Ty3/Gypsy retrotransposon, which was identified in the rice blast fungus Magnaporthe oryzae. Some Ty3/Gypsy retrotransposons, including MAGGY, contain a chromodomain‐like module (CLM) in the C‐terminus of the integrase domain. We have made a series of MAGGY mutants to examine the role of the CLM in the transposition activity of the element. Introduction of a mutation at different positions in the MAGGY integrase revealed that a loss or alteration of the CLM resulted in a drastic decrease in the transposition activity of the element. Our results indicate that the CLM may confer high transposition activity to the element.
Title: The C‐terminal chromodomain‐like module in the integrase domain is crucial for high transposition efficiency of the retrotransposon MAGGY
Description:
MAGGY is a Ty3/Gypsy retrotransposon, which was identified in the rice blast fungus Magnaporthe oryzae.
Some Ty3/Gypsy retrotransposons, including MAGGY, contain a chromodomain‐like module (CLM) in the C‐terminus of the integrase domain.
We have made a series of MAGGY mutants to examine the role of the CLM in the transposition activity of the element.
Introduction of a mutation at different positions in the MAGGY integrase revealed that a loss or alteration of the CLM resulted in a drastic decrease in the transposition activity of the element.
Our results indicate that the CLM may confer high transposition activity to the element.
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