Abstract 1410: Forced expression of EWS/ATF1 induces sarcomas in mice.

Abstract Clear cell sarcoma (CCS) is an aggressive soft tissue malignant tumor characterized by a unique t(12; 22) translocation, leading to the expression of a chimeric EWS/ATF1 fusion gene. However, little is known about the mechanisms underlying how EWS/ATF1 is involved in the development of CCSs. In addition, the cells of origin for CCSs remain to be determined. We generated EWS/ATF1-inducible mice, and examined the effects of EWS/ATF1 expression in adult somatic cells. We show that the forced expression of EWS/ATF1 results in the development of EWS/ATF1-dependent sarcomas in mice. The histology of EWS/ATF1-induced sarcomas resembles that of CCSs and EWS/ATF1-induced tumor cells express CCS-markers, such as S100, Sox10, and Mitf. Lineage tracing experiments using Wnt1-Cre and P0-Cre alleles indicated that neural crest-lineage cells are permissive to EWS-ATF1-driven transformation. Citation Format: Kazunari Yamada, Takatoshi Ohno, Akira Hara, Yasuhiro Yamada, Katsuji Shimizu. Forced expression of EWS/ATF1 induces sarcomas in mice. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1410. doi:10.1158/1538-7445.AM2013-1410