Pharmacognostic Evaluation of Simarouba glauca DC Bark

Simarouba glauca DC also known as ‘Dysentery Bark’ is an important ethnomedicinal plant native to Tropical America. Later introduced India as “Lakshmi-Taru.” Bark is a potent anti-microbial, anti-diabetic, anti-cancer, and hemolytic drug. This study presents a pharmacognostic evaluation of Simarouba glauca DC bark, mainly shedding light on its macroscopic and microscopic characteristics. Identification, authentication, macroscopic observation, and TLS, RLS and Powder microscopical examinations have been carried out. The macroscopic examination of the bark revealed distinctive features, including a rough, fissured surface, and a yellowish-brown colour. Microscopic analysis showcased the presence of a thick periderm with small flakes raised above the periderm surface. The outer part of phellem is crushed and the inner portion is modified into a thick cylinder of sclereids. The Phelloderm becomes thick walled and dark coloured and stores calcium oxalate crystals. Large prismatic calcium oxalate crystals located in uniseriate vertical row within thin parenchyma cells. TLS revealed that the phloem fibers in dense compact bundles. The fibers being highly thick walled and lignified with narrow cell lumen. The rays are 250-280 µm in height and 40-60µm. RLS showed the rays are rentered heterocellular. Parenchyma cells and fibers are vertical position at right angles to the rays. Bark powder microscopy exhibited the presence of narrow and wide fibers, fiber-sclereids, long sieve elements, thick brachy sclereids, and large quantity of quadrangular, rectangular, and polygonal Calcium oxalate crystals. These observations provide valuable diagnostic tools for the authentication and quality control of S. glauca DC bark. The pharmacognostic characterization presented in this study serves as a foundation for further research and development of S. glauca DC bark-based herbal medicines and pharmaceutical formulations. The insights provided in this article contribute to the understanding and utilization of this valuable botanical resource, fostering a bridge between traditional knowledge and contemporary scientific approaches in pharmacognosy.