Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Purification of 57kDa Hyaluronidase from the venom of Conus betulinus (Linnaeus, 1758)
View through CrossRef
Abstract
The enzyme hyaluronidase cleaves the substrate hyaluronic acid. In the study, hyaluronidase was isolated from the venom gland of C. betulinus and characterised using SDS-PAGE, FTIR, and HPLC. The protein content of crude venom is approximately 4mg/ml, whereas purification with Sepacryl S-100 yielded 0.04mg/ml protein with 0.463TRU/mg specific activity. The detected hyaluronidase had a molecular weight of 57kDa when compared to a standard protein marker. The presence of a peak at Rt 57.23 as hyaluronidase is revealed by HPLC analysis, and the wavelength pattern is similar to the standard bovine testicular hyaluronidase.
Title: Purification of 57kDa Hyaluronidase from the venom of Conus betulinus (Linnaeus, 1758)
Description:
Abstract
The enzyme hyaluronidase cleaves the substrate hyaluronic acid.
In the study, hyaluronidase was isolated from the venom gland of C.
betulinus and characterised using SDS-PAGE, FTIR, and HPLC.
The protein content of crude venom is approximately 4mg/ml, whereas purification with Sepacryl S-100 yielded 0.
04mg/ml protein with 0.
463TRU/mg specific activity.
The detected hyaluronidase had a molecular weight of 57kDa when compared to a standard protein marker.
The presence of a peak at Rt 57.
23 as hyaluronidase is revealed by HPLC analysis, and the wavelength pattern is similar to the standard bovine testicular hyaluronidase.
Related Results
Integrative multi-omics analysis reveals the contribution of neoVTX genes to venom diversity of Synanceia verrucosa
Integrative multi-omics analysis reveals the contribution of neoVTX genes to venom diversity of Synanceia verrucosa
Abstract
Background
Animal venom systems have been considered as valuable model for investigating molecular mechanisms underlying phenotypic evolution. The stonefish were ...
Hyaluronidase in Clinical Isolates ofPropionibacterium acnes
Hyaluronidase in Clinical Isolates ofPropionibacterium acnes
Objectives. We sought to describe the prevalence of a hyaluronidase gene and hyaluronidase production in 197 clinical isolates ofP. acnes; we assessed kinetics of hyaluronidase pro...
EVALUATION OF BARU (DIPTERYX ALATA) FLOUR AS A POTENTIAL INHIBITOR OF THE LACHESIS MUTA MUTA SNAKE VENOM -INDUCED MYOTOXICITY AND NEUROMUSCULAR BLOCKADE
EVALUATION OF BARU (DIPTERYX ALATA) FLOUR AS A POTENTIAL INHIBITOR OF THE LACHESIS MUTA MUTA SNAKE VENOM -INDUCED MYOTOXICITY AND NEUROMUSCULAR BLOCKADE
Snake venom is a complex mixture of bioactive substances known to induce a wide range of toxic effects. We investigated the potential of baru flour (Dipteryx alata) in mitigating t...
Pre-clinical Efficacy and Immunogenicity of IgY Antibodies Directed
against Crotalus durissus cumanensis Venom
Pre-clinical Efficacy and Immunogenicity of IgY Antibodies Directed
against Crotalus durissus cumanensis Venom
Background:
Crotalus durissus cumanensis (C.d.c.) is the most widely distributed snake in Venezuela, causing the majority of snakebite envenoming
Objective:
The purpose of this s...
Comparative venom gland transcriptome analysis of the scorpion Lychas mucronatus reveals intraspecific toxic gene diversity and new venomous components
Comparative venom gland transcriptome analysis of the scorpion Lychas mucronatus reveals intraspecific toxic gene diversity and new venomous components
Abstract
Background
Lychas mucronatus is one scorpion species widely distributed in Southeast Asia and southern China. Anything is...
Viper Venom and Synthetic Peptides: Emerging Active Ingredients in Anti-Ageing Cosmeceuticals
Viper Venom and Synthetic Peptides: Emerging Active Ingredients in Anti-Ageing Cosmeceuticals
The animal kingdom, particularly reptiles, is widely recognized as a valuable source of peptides and proteins with applications in medicine, the pharmaceutical industry and, more r...
Lectin C gene analysis v1
Lectin C gene analysis v1
Mammalian Tissue Total RNA Purification Protocol by GeneJET RNA Purification Kit (Thermo Scientific, USA) Before starting: • Supplement the required amount of Lysis Buffer with β-...
Visualisation of expanded conus branch for overfilled with contrast medium
Visualisation of expanded conus branch for overfilled with contrast medium
Case Study
A 74-year-old male was admitted with atypical chest pain. Transradial coronary angiography revealed that there was no obstructive disease in left anterior...