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Screening of diacetyl producing Lactobacillus isolates isolated from raw cow milk
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In the present study, diacetyl production by isolates of Lactobacillus was examined. Lactobacillus isolates were isolated from raw cow milk. Altogether 13 isolates were isolated from milk out of which 4 isolates belongs to L. acidophilus, 3 belongs to L. brevis, 2 belongs to L. casei, 2 from L. fermentum and 2 from L. rhamnosus. Diacetyl production was tested in isolates of L. casei, L. brevis and L. rhamnosus at different pH, temperature and multiple susbtrate. All isolates produced maximum diacetyl at pH 6.5. Effect of temperature on diacetyl production varied in different isolates. Isolates of L. casei produced maximum 0 0 diacetyl at a temperature of 8 C, isolates of L. brevis produced maximum diacetyl at a temperature of 18 C 0 and isolates of L. rhamnosus produced maximum diacetyl at a temperature of 20 C. Glucose, lactose and citrate were used as substrate and diacetyl production by isolates was tested. Maximum diacetyl production was observed when lactose and citrate were supplied as substrate.. KEYWORDS :Lactobacillus, Diacetyl, pH, temperature, glucose, lactose, citrate.
Title: Screening of diacetyl producing Lactobacillus isolates isolated from raw cow milk
Description:
In the present study, diacetyl production by isolates of Lactobacillus was examined.
Lactobacillus isolates were isolated from raw cow milk.
Altogether 13 isolates were isolated from milk out of which 4 isolates belongs to L.
acidophilus, 3 belongs to L.
brevis, 2 belongs to L.
casei, 2 from L.
fermentum and 2 from L.
rhamnosus.
Diacetyl production was tested in isolates of L.
casei, L.
brevis and L.
rhamnosus at different pH, temperature and multiple susbtrate.
All isolates produced maximum diacetyl at pH 6.
5.
Effect of temperature on diacetyl production varied in different isolates.
Isolates of L.
casei produced maximum 0 0 diacetyl at a temperature of 8 C, isolates of L.
brevis produced maximum diacetyl at a temperature of 18 C 0 and isolates of L.
rhamnosus produced maximum diacetyl at a temperature of 20 C.
Glucose, lactose and citrate were used as substrate and diacetyl production by isolates was tested.
Maximum diacetyl production was observed when lactose and citrate were supplied as substrate.
KEYWORDS :Lactobacillus, Diacetyl, pH, temperature, glucose, lactose, citrate.
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