A Comparison of Bicarbonate Hemodialysis, Hemodiafiltration, and Acetate‐free Biofiltration on Cytokine Production

Abstract:  Acetate‐free biofiltration (AFB) is a special hemodiafiltration (HDF) modality performed with a base‐free dialysate and simultaneous injection of non‐pyrogenic sodium bicarbonate solution. The purpose of this study was to investigate the difference of cytokine production by conventional bicarbonate hemodialysis (BCD), standard HDF and AFB in the same patients. Eight stable hemodialysis patients were treated in random order with BCD, HDF and AFB every 4 weeks. The production of interleukin‐1β (IL‐1β) and interleukin‐1 receptor antagonist (IL‐1Ra) by peripheral blood mononuclear cells (PBMC) was investigated without stimulation and with stimulation by a small amount of endotoxin (ET)‐contaminated β2‐microglobulin (β2M) and lipopolysaccharide (LPS) before and after dialysis treatment in the last sessions during all periods. To serve as controls, 14 healthy volunteers participated in this study. In spontaneous IL‐1Ra production, the values of before and after AFB were not significantly different from that of the controls, and the values of before and after BCD and before HDF were significantly higher than that of the controls. In LPS‐stimulated PBMC, IL‐1β production before and after AFB was not significantly different from that of the controls, and before and after BCD and HDF was significantly higher than that of the controls. In ET‐contaminated β2M‐stimulated PBMC, IL‐1β production before and after AFB was not significantly different compared to the controls, and the production was significantly lower than that before and after BCD and HDF. In addition, IL‐1Ra production after AFB was not significantly different from the controls, and the production was significantly lower than that before and after BCD and HDF. It was concluded that a lower cytokine production by AFB may have the effect of preventing dialysis‐related complications.