Supplementary Figures S1 - S9 from HIF2α-Dependent Lipid Storage Promotes Endoplasmic Reticulum Homeostasis in Clear-Cell Renal Cell Carcinoma

<p>Supplementary Figures S1 - S9: Supplementary Figure S1. Regulation of PLIN2 and other LD coat protein genes in ccRCC tissue. Supplementary Figure S2. PPARγ function is not required for PLIN2 expression in ccRCC. Supplementary Figure S3. HIF-2alpha dependent PLIN2 expression promotes cell viability in nutrient deprived regions of 3D tumor spheroids. Supplementary Figure S4. PLIN2 is required for lipid storage, cell viability, and maintenance of ER homeostasis in A498 cells. Supplementary Figure S5. Validation of IRE-1alpha inhibitor, PERK inhibitor, and ATF6 siRNA in A498 cells. Supplementary Figure S6. Suppression of UPR signaling in A498 cells following PLIN2 depletion. Supplementary Figure S7. Energetic effects of PLIN2 depletion in ccRCC cell lines and evidence for suppression of beta-oxidation in ccRCC tumor tissue. Supplementary Figure S8. UPR or fatty acid synthesis modulation in PLIN2 depleted cells. Supplementary Figure S9. Lipid storage protects against brefeldin A induced ER stress.</p>