Frequency-dependent changes in the activity of blood enzymes under the action of modulated ultrasound.

The subject of the study is frequency-dependent changes in the activity of blood enzymes of laboratory animals (rats) under the influence of modulated ultrasound of a therapeutic range of intensities. With equal-energy exposure to the enzyme systems under study, action spectra are shown for the following enzymes: creatine kinase, alkaline phosphatase, lactate dehydrogenase, aspartate aminotransferase, cholinesterase, indicating the possibility of frequency control of the functional state of enzymes, activation and inhibition of enzyme activity at frequencies specific for each enzyme. The activity of all enzymes is controlled in two ways: a) by changing the frequency of the influencing signal and b) by changing the energy (or amplitude) of the influencing signal at the biologically active frequency. Obtaining detailed action spectra is a necessary condition for developing the theoretical basis for determining biologically active frequencies and mechanisms of frequency-dependent responses of specific biological systems to external influences of modulated waves. The main conclusions of the study are: The main approach to studying modulated waves is to obtain and use action spectra for various biological systems. Using exposure at an active frequency, it is possible, by changing the energy of the impacting ultrasound, to obtain multidirectional effects. Thus, both the modulation frequency and the impact energy can be factors in controlling the functional state of enzyme systems using modulated ultrasonic waves. The purpose of the article is to show the presence of frequency-dependent responses of various enzyme systems when exposed to modulated ultrasonic waves of a therapeutic range of intensities - performed using the example of five enzyme systems. The action spectra for these systems and the possibility of transforming these spectra when changing the ultrasound intensity are presented. The possibility of controlling the magnitude and sign of the responses of the studied enzyme systems, both the modulation frequency and the energy of ultrasonic influence, has been demonstrated.