Application of Immunohistochemistry to Monitor the CD3+T Cell Subset Counts in Patients with Sepsis

Abstract [Objective]: To evaluate the application significance of Immunohistochemistry for monitoring peripheral blood CD3 + T cell subset (CD3+/CD3 + CD4+/CD3 + CD8+) counts in patients with sepsis.[Methods]: Two peripheral blood samples of 117 patients with sepsis on the first day of admission (D1) and 20 healthy control subjects were collected, and two peripheral blood samples of 20 patients with sepsis on the fourth day of admission (D4) were randomly collected and used to detect the lymphocyte counts of routine blood tests and CD3 + T cell subset count by Immunohistochemistry; the lymphocyte count levels between the sepsis group and the healthy control group were compared, and the correlation between the two in the same group were analyzed.[Results]:lymphocyte counts by routine blood tests and CD3 + T cell subset counts of patients with sepsis were significantly lower than those in healthy control subjects (P < 0.01). In the surviving group, the mean values of D4 CD3 + T cell subset counts increased significantly compared with D1, while the nonsurviving group did not rebound significantly; There was a significant positive correlation between lymphocyte counts by routine blood tests and CD3 + T lymphocyte subset counts in patients with sepsis and the healthy control subjects. (P < 0.01).[Conclusion]: Detection of CD3 + T cell subset counts by immunohistochemical method can reflect the cellular immune status of patients at a given time, thus it can be used as one of the immune monitoring methods in patients with sepsis.