Search engine for discovering works of Art, research articles, and books related to Art and Culture
Javascript must be enabled to continue!
Micro-liquid competent cells — a simple and practical protocol for the preparation of competent cells for DNA recombination v1
View through CrossRef
Cellular transformation with recombinant DNA is an important technique in molecular biology. Weestablished a simple and practical protocol for preparing high-quality competent Escherichia coli cells within a few hours. We named these cells micro-liquid competent (mLC) cells because they are prepared in microtubes and stored as a liquid at 4oC. Competent cells prepared with this protocol may be used for several weeks for plasmid DNA recombination.
Springer Science and Business Media LLC
Title: Micro-liquid competent cells — a simple and practical protocol for the preparation of competent cells for DNA recombination v1
Description:
Cellular transformation with recombinant DNA is an important technique in molecular biology.
Weestablished a simple and practical protocol for preparing high-quality competent Escherichia coli cells within a few hours.
We named these cells micro-liquid competent (mLC) cells because they are prepared in microtubes and stored as a liquid at 4oC.
Competent cells prepared with this protocol may be used for several weeks for plasmid DNA recombination.
Related Results
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Abstract
Background: Age-associated epigenetic alteration is the underlying cause of DNA damage in aging cells. Two types of youth-associated DNA-protection epigenetic mark...
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Genome wide hypomethylation and youth-associated DNA gap reduction promoting DNA damage and senescence-associated pathogenesis
Introduction: The United States currently faces two opioid crises, an evolved crisis currently manifesting as widespread abuse of illicit opioids, and a crisis in pain management l...
The influence of micro influencers and digital marketing on product purchasing decisions at tiktok shop in bengkulu city
The influence of micro influencers and digital marketing on product purchasing decisions at tiktok shop in bengkulu city
THE INFLUENCE OF MICRO-INFLUENCERS AND DIGITAL MARKETING ON PURCHASE DECISIONS OF TIKTOK SHOP CUSTOMERS IN BENGKULU CITY
Andhes Tiani Putri, Meylaty F
12Faculty Of Economic
E...
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing v1
Human tissues comprise trillions of cells that populate a complex space of molecular phenotypes and functions and that vary in abundance by 4–9 orders of magnitude. Relying solely ...
Echinococcus granulosus in Environmental Samples: A Cross-Sectional Molecular Study
Echinococcus granulosus in Environmental Samples: A Cross-Sectional Molecular Study
Abstract
Introduction
Echinococcosis, caused by tapeworms of the Echinococcus genus, remains a significant zoonotic disease globally. The disease is particularly prevalent in areas...
Successful transfection of Lymphoblastoid cell line (Preprint)
Successful transfection of Lymphoblastoid cell line (Preprint)
BACKGROUND
Immortalization is the stage that the cell goes through before full transformation [1]. Human resting B lymphocytes from peripheral blood are eas...
Topoisomerase Assays
Topoisomerase Assays
AbstractTopoisomerases are enzymes that play essential roles in DNA replication, transcription, chromosome segregation, and recombination. All cells have two major forms of DNA top...
EWOD Based Liquid-Liquid Extraction and Separation
EWOD Based Liquid-Liquid Extraction and Separation
Liquid-liquid extraction techniques are one of the major tools in chemical engineering, analytical chemistry, and biology, especially in a system where two immiscible liquids have ...