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Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
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AbstractBar-headed geese (Anser indicus) are adaptable to plateau environments. In this study, we sequenced and assembled a high-quality chromosome-level genome of the bar-headed goose using PacBio long reads and Hi-C technique, and generated 115.73 Gb of Illumina short-reads and 95.89 Gb of PacBio long-reads. The assembled bar-headed goose genome, with a contig N50 of 5.734 Mb and a scaffold N50 of 65.77 Mb, is 1.129 Gb in length and includes 33 chromosomes and 451 fragments. BUSCO assessment yielded a completeness score of 94.4%. In total, 15,376 protein-coding genes were predicted, of which 94.95% had homologs in protein databases. We identified 78 positively selected genes (PSGs) in the bar-headed goose genome, which were mainly enriched in calcium ion and ATP-binding. This bar-headed goose genome will be an important resource for increasing our understanding regarding the genetic basis of adaptation to life at a high altitude.
Springer Science and Business Media LLC
Title: Chromosome-level genome assembly of the bar-headed goose (Anser indicus)
Description:
AbstractBar-headed geese (Anser indicus) are adaptable to plateau environments.
In this study, we sequenced and assembled a high-quality chromosome-level genome of the bar-headed goose using PacBio long reads and Hi-C technique, and generated 115.
73 Gb of Illumina short-reads and 95.
89 Gb of PacBio long-reads.
The assembled bar-headed goose genome, with a contig N50 of 5.
734 Mb and a scaffold N50 of 65.
77 Mb, is 1.
129 Gb in length and includes 33 chromosomes and 451 fragments.
BUSCO assessment yielded a completeness score of 94.
4%.
In total, 15,376 protein-coding genes were predicted, of which 94.
95% had homologs in protein databases.
We identified 78 positively selected genes (PSGs) in the bar-headed goose genome, which were mainly enriched in calcium ion and ATP-binding.
This bar-headed goose genome will be an important resource for increasing our understanding regarding the genetic basis of adaptation to life at a high altitude.
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