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Prevalence of Community Acquired Methicillin Resistant Staphylococcus Aureus (Ca-Mrsa) In The Nasal Cavity of Delta State University Students.

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INTRODUCTION Staphylococcus aureus (SA) is a Gram positive, opportunistic bacterium that frequently colonizes the oral cavity, nasal cavity, and skin of the healthy people. This can cause a variety of localized and invasive problems ranging from superficial skin infections to life threatening Pneumonia and bloodstream infections OBJECTIVE: The study is aimed at investigating the prevalence of community acquired methicillin resistant Staphylococcus aureus in the nasal cavity of Delta State University students METHOD:100 nasal swabs(samples) were collected from the anterior nares of both male and female students of DELSU using Sterile swab sticks and was cultured on mannitol salt Agar .After culturing on mannitol salt Agar, all the Staphylococcus aureus specie were isolated.Standard identification test was carried out by performing Gram staining test and other biochemical test like coagulase test, Catalase test and Fermentation test were further used to determine the identity of the organism. Antibiotic susceptibility test was carried out for Staphylococcus aureus using the Agar well diffusion method.The zone of inhibition in millimeters was measured with a meter rule. Theaverage readings were taken to be the zone of inhibition and were compared with KirbyBauer Standard for Staphylococcus aureus. zones of inhibitions within the range(≤10)were used to determine the Methicillin resistant Staphylococcus aureus(MRSA). RESULT: Out of the 100 samples collected and cultured on the mannitol salt agar, 93 were staph species why 33(35.5%) were found to be Staphylococcus aureus why the remaining 60(64.5%) were other specie of Staphylococcus aureus .Only the staph aureus specie were worked on and out of the 33(35.5%) Staphylococcus aureus, 23(69.7%) of the Staphylococcus aureus were found to be methicillin resistant why 10(30.3%) were found to be sensitive to Oxacillin disk which was used as a reference to methicillin antibiotics because its closely related to methicillin. CONCLUSION: The result from this study shows increased Staphylococcus aureusresistance to methicillin(Oxacillin),therefore community acquired methicillin resistant Staphylococcus aureus is prevalent in the nasal cavity of DELSU Students and therefore suggest that more work has to be done as an individual and even in the clinical setting and in the community to curtail the spread of methicillin resistant Staphylococcus aureus.
Title: Prevalence of Community Acquired Methicillin Resistant Staphylococcus Aureus (Ca-Mrsa) In The Nasal Cavity of Delta State University Students.
Description:
INTRODUCTION Staphylococcus aureus (SA) is a Gram positive, opportunistic bacterium that frequently colonizes the oral cavity, nasal cavity, and skin of the healthy people.
This can cause a variety of localized and invasive problems ranging from superficial skin infections to life threatening Pneumonia and bloodstream infections OBJECTIVE: The study is aimed at investigating the prevalence of community acquired methicillin resistant Staphylococcus aureus in the nasal cavity of Delta State University students METHOD:100 nasal swabs(samples) were collected from the anterior nares of both male and female students of DELSU using Sterile swab sticks and was cultured on mannitol salt Agar .
After culturing on mannitol salt Agar, all the Staphylococcus aureus specie were isolated.
Standard identification test was carried out by performing Gram staining test and other biochemical test like coagulase test, Catalase test and Fermentation test were further used to determine the identity of the organism.
Antibiotic susceptibility test was carried out for Staphylococcus aureus using the Agar well diffusion method.
The zone of inhibition in millimeters was measured with a meter rule.
Theaverage readings were taken to be the zone of inhibition and were compared with KirbyBauer Standard for Staphylococcus aureus.
zones of inhibitions within the range(≤10)were used to determine the Methicillin resistant Staphylococcus aureus(MRSA).
RESULT: Out of the 100 samples collected and cultured on the mannitol salt agar, 93 were staph species why 33(35.
5%) were found to be Staphylococcus aureus why the remaining 60(64.
5%) were other specie of Staphylococcus aureus .
Only the staph aureus specie were worked on and out of the 33(35.
5%) Staphylococcus aureus, 23(69.
7%) of the Staphylococcus aureus were found to be methicillin resistant why 10(30.
3%) were found to be sensitive to Oxacillin disk which was used as a reference to methicillin antibiotics because its closely related to methicillin.
CONCLUSION: The result from this study shows increased Staphylococcus aureusresistance to methicillin(Oxacillin),therefore community acquired methicillin resistant Staphylococcus aureus is prevalent in the nasal cavity of DELSU Students and therefore suggest that more work has to be done as an individual and even in the clinical setting and in the community to curtail the spread of methicillin resistant Staphylococcus aureus.

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