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Range of Molecular Weight of Different Effective Components in the Aqueous Extract of Angelica-Radix Hedysari Ultrafiltration Content by HPLC-MS
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The molecular weight range of effective components in the aqueous extract of Angelica-Radix Hedysari (1:5) was analyzed, providing a foundation for understanding its chemical composition and pharmacodynamics. Ultrafiltration technology was used to prepare different forms of the extract, including water extract powder, ultrafiltration powder, ultrafiltration saponin component powder, and ultrafiltration polysaccharide component powder. High-performance liquid chromatography-mass spectrometric (HPLC-MS) were used to analyze these extracts. Comparison between the components of Angelica-Radix Hedysari using HPLC and HPLC-MS showed that the ultrafiltration products contained fewer components than the original extract powder. However, the content of some components in the ultrafiltration products increased. The components with molecular weight greater than 10 kDa were removed during ultrafiltration, resulting in fewer components in the ultrafiltration products compared to Angelica Hongqi extract powder.
Title: Range of Molecular Weight of Different Effective Components in the Aqueous Extract of Angelica-Radix Hedysari Ultrafiltration Content by HPLC-MS
Description:
The molecular weight range of effective components in the aqueous extract of Angelica-Radix Hedysari (1:5) was analyzed, providing a foundation for understanding its chemical composition and pharmacodynamics.
Ultrafiltration technology was used to prepare different forms of the extract, including water extract powder, ultrafiltration powder, ultrafiltration saponin component powder, and ultrafiltration polysaccharide component powder.
High-performance liquid chromatography-mass spectrometric (HPLC-MS) were used to analyze these extracts.
Comparison between the components of Angelica-Radix Hedysari using HPLC and HPLC-MS showed that the ultrafiltration products contained fewer components than the original extract powder.
However, the content of some components in the ultrafiltration products increased.
The components with molecular weight greater than 10 kDa were removed during ultrafiltration, resulting in fewer components in the ultrafiltration products compared to Angelica Hongqi extract powder.
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