Anti-depressant Effect of Fluoxetine on Brain Indolamine Levels to Improve the RA Associated Depression in Adjuvant Induced Arthritic Rats

Background: Rheumatoid arthritis (RA) is a neuro-immune disorder which causes neurological problem majorly depression along with joints soreness. Reduced brain indolamines level is one the reason among them Objectives: The core objective of this research is to treat the RA associated depressive arthritic rats with fluoxetine (very effective SSRIs). For this purpose, chronic arthritis was induced in healthy female rats by adjuvant. Brain indolamines (BI) levels (Tryptophan, serotonin and HIAA) were estimated in three groups, one is of healthy rats having no arthritis i.e. No AIA (positive control), second adjuvant-induced arthritic (AIA) rats that act as negative control and third is test group i.e. (Fluox AIA) AIA rats receiving fluoxetine (10mg/Kg, orally). As decrease concentration of BI contribute to make body depressed and elevated levels of theses markers by fluoxetine helps to improve the depressant condition in AIA rats. Methodology: Adjuvant induced arthritic rat (AIA) model were used in this study which having same pathological conditions as RA patients possess. To induce arthritis1 mg of fresh lyophilized Mycobacterium tuberculosis H37Ra (MT H37Ra; DIFCO Laboratories, Detroit, MI, USA) in 0.1 ml of liquid paraffin oil was injected into the hind paw of all female Sprague-Dawley rats. Rats were grouped into three categories i.e. positive control (having no arthritis), AIA negative control and AIA Fluox (receiving fluoxetine). Change in paw volumes was assessed to observe the arthritic progression. Levels of BI were estimated through HPLC-EC technique to determine anti-depressant effect of fluoxetine. Data was analyzed by one way ANOVA using Tukey’s post hoc analysis. Results: Major increase (p<0.005) were found in all BI in arthritic rats receiving fluoxetine when compared against AIA control group. Conclusion: Brain indolamines level increased significantly in AIA rats receiving fluoxetine when compared with control which help them to improve depressant condition associated with RA.