Estimation of Total Flavonoids and Antioxidant Activity of Spilanthes acmella Leaves

Flavonoids are a group of polyphenolic compounds, which are widely distributed throughout the plant kingdom. Flavonoids of different classes exhibited various pharmacological and biological activities. The present study was undertaken to investigate the total flavonoids and antioxidant effect of Spilanthes acmella leaves. The ethanol extract of Spilanthes acmella leaves were prepared and performed its phytochemical screening. The total flavonoids and polyphenol were investigated to quantify the presence of polyphenol compounds. The 2,2-diphenyl-1-picryl-hydrazil stable radical (DPPH) and Superoxide scavenging radical were used to determine extract antioxidant activity. The concentrations of flavonoids polyphenol in ethanol extract of Spilanthes acmella were 72.14 QE mg/gm and 84.52 GAE mg/gm, respectively.  The extract exhibited the strongest antioxidant activity, with the lowest IC50 ­value for DPPH and Superoxide scavenging. The IC50 ­value for DPPH and Superoxide scavenging were 134.11 µg/ml and 104.51 µg/ml, respectively.   The strongest antioxidant activity of ethanol extract could be due to the presence of flavonoids and phenols.