Background: Nowadays, some bacterial infections have experienced resistance to antibiotic drugs which is a major challenge in public health throughout the world. The evolution of resistance in microorganisms can cause some existing antibiotic drugs to become less effective in the treatment of infections caused by microorganisms. One of the natural plant materials that can provide an antibacterial effect is Mango leaves or with the Latin name M. indica L. which comes from the Anacardiaceae family. Objective: To determine whether the ethyl acetate extract of mango leaves has antibacterial activity against S. aureus and S. epidermidis. Design: Mango leaf extract (M. indica) was obtained by maceration method using Ethyl Acetate solvent. The extract obtained was then subjected to antibacterial testing of the test bacteria using the diffusion method with various concentrations of 2.5%, 5%, 10% (w/v), ampicillin as positive control and 10% DMSO as negative control. The inhibition zone formed was measured after 24 hours of incubation.. Results: There were inhibition zones in the antibacterial test of the ethyl acetate extract of mango (M. indica) leaves against S. aureus of 7.6 mm, 8 mm, 8.7 mm and 27.3 for the positive control. For antibacterial testing of mango leaf ethyl acetate extract (M. indica) against S. epidermidis of 7.8 mm, 8.4 mm, 8.6 mm and 23.3 for positive control. Conclusions: Based on the tests that have been carried out, it can be concluded that the ethyl acetate extract of mango (M. indica) leaves has antibacterial compounds.