Preliminary analysis and validation of the functions associated with two related genes during Beta-Aminobutyric acid induced resistance in blueberry against to leaf spot

Abstract Background: Blueberry leaf spot is one of the most important diseases associated with blueberry production. However, little research has been done on blueberry interactions with leaf spot and blueberry resistance to leaf spot. Methods and Results:In this study, the induced blueberry with BABA was inoculated with the leaf spot pathogen as the experimental group, and blueberry inoculated with the uninduced leaf spot pathogen was used as a control group to construct a transcriptome sequencing library. A total of 11 disease-resistant genes were randomly screened from the transcription library PHI database for qRT-PCR verification, and two disease-resistant genes containing comp126500_c0_seq1and comp126556_c0_seq2 were selected from eight genes with good amplification specificity in order to elucidate the function of blueberry leaf spot resistance genes. Cloning the cDNA sequences of the two genes using qRT-PCR and RACE; analysis of biological information by amino acid sequencing using two disease-resistant protein gene sequences of amino acids to build an evolutionary tree; and genetic compound conservative domains and protein secondary and tertiary structure predictions, revealed the fungus stress resistance related gene function during the early response to blueberry leaf spot. Using agrobacterium mediated transformation of Arabidopsisexpression to understand two gene's defensive roles during leaf spot infection. Conclusions: The results preliminarily proved that comp126500_c0_seq1and comp126556_c0_seq2, which produced in the body of a blueberry by induction of BABA, are related genes with resistance to leaf spot.