Proliferation of cells of the pancreas in experimental diabetes

The proliferative process intensification in the disrupted organs is very important in reparative regeneration. But the possibility of pancreas cells proliferation during damage is not been studied. The aim of our work was to examine the proliferation activity of pancreas during the experimental alloxan diabetes. The work was made on 33 rats. Blood glucose levels were measured. Also the expression of nuclear antigen of proliferating cells PCNA was studied in pancreas cells. After the day of the experimental hyperglycemia PCNA expression in islet and acinar cells of pancreas was found, which was persisted at all stages of the experiment. After 2 days of the experiment single PCNA-positive cells with the cytoplasmic staining were found in the interstitium, near vessels and ducts. Proliferating cells, which produce insulin were found in islets during double staining. Thus, there is the proliferation in islet and acinar cells of pancreas during experimental diabetes type I after alloxan damage. Also, the results of our study confirms the ability of p-cells and progenitor pancreas cells proliferation.